Eponate 12™ Embedding Kit, with BDMA
Includes:
Eponate 12™ Resin, 450g
DDSA, 450g (18022)
NMA, 450g (18032)
BDMA, 25g
Aliphatic epoxy resin based on di- and tri-glydicyl ethers of glycerol with a significantly lower viscosity compared to Araldite 502. BDMA (<3%, see notes preceding) is used as the accelerator since it has lower viscosity and diffuses into tissues more rapidly. (See references below).
The viscosity of Eponate™ 12 at 25ºC is 15-21cP, WPE is 140–145 and Specific Gravity is 1.15–1.22g/ml. Other kit viscosities at 25ºC include DDSA (290cP) and NMA (225-315cP). Eponate™ 12 is hygroscopic.
Curing time is 16 to 24 hours at a temperature of 60°C or 2 to 3 hours using the PELCO BioWave®.
First use of Epon 812 in U.S.A.:
Finck H, 1960. Epoxy resins in electron microscopy. J Biophys Biochem Cytol, 7, p. 27.
Luft JH, 1961. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol, 9, p. 409.